Vol. 9 No. (2): 83 - 92 (2018): EFFECTS OF MANGIFERA INDICA EXTRACT ON HAEMATOLOGICAL AND HISTOPATHOLOGICAL INDICES IN LABORATORY MICE
Toxicological properties of crude leaf extract of Mangifera indica were carried out using
standard procedures in laboratory mice. Forty Swiss albino mice grouped into four groups
each for the acute and sub-acute toxicity study were administered doses of 100, 200,
300mg/kg and normal saline (control). The results of showed increased in weight from
21.54±0.95g to 29.53±0.48g, 21.45±0.84g to 29.16±3.33g, 21.23±0.90g to 23.81±1.28g
for 300mg/kg, 200mg/kg and 100mg/kg respectively after 14 days of treatment. The mice
treated with 300mg/kg progressively increased in weight from 18.52±0.48 to 26.93±0.74g
after 21days (week 3), the 200mg/kg treated group increased from 19.14±0.98 to
30.76±0.56g in week 4 while the 100mg/kg treated group increased from 19.46±0.76 to
30.23±0.45 in week 3 respectively after 28 days of treatment. The haemoglobin counts
were 10.15±0.35g/dl, 10.78±0.50g/dl, 9.78±0.33g/dl and 10.12±0.32g/dl for 300, 200,
100mg/kg and control groups respectively. The packed cell volume (PCV) increased from
28.00±2.09% control group to 28.80±0.97%, 32.50±1.85% and 29.25±2.14% for 100, 200
and 300mg/kg respectively. Furthermore, the red blood cell (RBC) counts also increased
from 3.82±0.29x1012/L to 4.33±0.35 x 1012/L for 200mg/kg. There was a general increase
in the white blood cell (WBC) levels 5.36±1.03 x 109/L, 6.21±0.78 x 109/L, and 5.84±1.13
x 109/L for 100, 200 and 300mg/kg compared to the control 3.98±0.55 x 109/L. The mean
corpuscular volume (MCV) revealed an increase that is dose dependent 7.56±0.84,
8.05±1.00 and 8.12±0.79cuµ for 100, 200 and 300mg/kg respectively while the mean
corpuscular haemoglobin (MCH) 2.57±0.30, 2.56±0.28 and 2.84±0.29pg did not follow
this pattern and the mean corpuscular haemoglobin concentration (MCHC) 34.04±0.32,
33.23±0.81 and 33.04±1.01% for 100, 200 and 300mg/kg decreased respectively. The
histophatological defects observed were enlargement of nuclei on the liver and necrosis of
the glomeruli of the kidney administered with higher doses of the kidney.
Keywords: Mangifera indica, haematological indices, histopathological, albino mice.